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It is very important for the physicians to know what bacteria cause diseases so that they are able to prescribe a narrow spectrum antibiotic, rather than prescribing a broad spectrum antibiotic. Mosby’s Medical Dictionary, 8th edition.There are many practical and theoretical applications for identifying unknown bacteria. Vulgaris are ampicillin and aminoglycosides. Antimicrobials that are most effective in inhibiting P. Grow well in the intestinal tract, thriving in the urinary tract instead. vulgaris is an enteric bacteria, is does not vulgaris is commonly associated with urinary, It is commonly found in fecal andĭecomposing matter, soil, and water. vulgaris part of the Enterobacteriaceae family. vulgaris grew in clear colonies, fermenting xylose but not producing To confirm, theīacterium was plated on a desoxycholate plate as a differential test for P. A urea test, testing for the activated enzyme urease was Negative VP result indicated the bacterium did not produce acetyl methyl carbinol and could onlyīe Escherichia coli or P. Narrowed the possible bacterium down to three with a Vogues-Proskauer test following. A positive methyl red test indicated acid production during glucose fermentation and **Flowcharts in RESULTS was removed due to formatting reasons.Īfter a Gram negative pure culture was assigned because of a contaminated mixed culture, aĬareful order of tests was followed that led to Proteus vulgaris as the unknown from the alternateīacterium. The following tests were performed on Gram negative alternate culture and are documented on Negative pure culture following its growth on a nutrient agar plate and a Gram stain to confirm it
#PROTEUS VULGARIS GRAM STAIN MANUAL#
The following tests were performed on Gram positive culture “B” and are documented onĪn alternate Gram negative was then randomly assigned as Alternate # 3 and methods and testsįrom the lab manual were used to determine its identity. Culture “B” was then determined to be a Gram positiveīacillus based on Gram staining and was an uncontaminated pure culture.
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Based on the tests it was not determined what the identity of Culture “A” After repeating Gram stains for both cultures it was determined that both cultures Fermentation Tubes for glucose, galactose, and maltoseĪfter completing the above tests for both cultures the identity of the unknown bacteria was still
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The following tests were performed on Gram negative culture “B” and are documented onħ. Note: The results from the above test were inconclusive, reflected in the chart. The following tests were performed on Gram positive culture “A” and are documented on Positive cocci and culture “B” was a Gram negative rod. Then performed on the two different cultures and it was determined that culture “A” was a Gram Nutrient agar plate, culture “A” was re-plated to isolate a more pure culture. Grown on separate nutrient agar plates labeled cultures “A” and “B”. Two different cultures grew and were visibly observed, isolated, and The first procedure completed was the growth of two isolation streaks on nutrient agar platesįrom mixed culture 102. Procedures outlined in the lab manual were followed. Outlined and practiced from Lab Manual for General Microbiologyi were used in theĭetermination of unknown bacteria from tube 102 as well as an alternate Gram negativeīacterium. Patients, surfaces and foods using varied laboratory techniques.Īn unknown bacteria tube, number 102, was distributed randomly by course instructor. The practical application of this study includes determining unknown bacteria in/on Laboratory study was an exercise in identifying two bacterium using varied scientific techniques The discipline of microbiology has many applications in science and medicine.